Publications – Dr Galkin's lab

Panconesi R, Carvalho M, Eden J, Fazi M, Ansari F, Mancina L, Navari N, Sousa Da Silva R, Dondossola D, Borrego L, Pietzke M, Peris A, Meierhofer D, Muiesan P, Galkin A, Marra F, Dutkowski P, Schlegel A. (2023) Mitochondrial injury during normothermic regional perfusion (NRP) and hypothermic oxygenated perfusion (HOPE) in a rodent model of DCD liver transplantation. EBioMedicine PMID: 329224707 PDF
Yoval-Sánchez B, Ansari F, Lange D, Galkin A. (2022) Effect of metformin on intact mitochondria from liver and brain: Concept revisited. Eur J Pharmacol. 175177. PMID: 35934089 PDF
Yoval-Sánchez B, Ansari F, James J, Niatsetskaya Z, Sosunov S, Filipenko P, Tikhonova IG, Ten V, Wittig I, Rafikov R, Galkin A. (2022) Redox-dependent loss of flavin by mitochondria complex I is different in brain and heart. Redox Biol. 51, 102258. PMID: 35189550 PDF
Ansari F, Yoval-Sanchez B, Niatsetskaya, Ten V, Wittig I, Galkin A. (2022) How many molecules of mitochondrial complex I are in a cell? Analyt. Biochem. 114646, PMID: 35259403 PDF
Suslova TA, Vavilov MN, Belyaeva SV, Evdokimov AV, Stashkevich DS, Galkin A, Kofiadi IA. (2022) Distribution of HLA-A, -B, -C, -DRB1, -DQB1, -DPB1 allele frequencies in patients with COVID-19 bilateral pneumonia in Russians, living in the Chelyabinsk region. Human Immunol. S0198-8859(22)00090-8. PMID: 35525710 PDF
Krokhin AA, Rusakov MN, Belyaeva SV, Galkin A, Suslova TA. (2022) ABO blood type analysis of the donors of convalescent plasma after COVID-19 infection in Chelyabinsk region, Russia. Vox Sang, 117, 457. PMID: 34268789 PDF
Průchová P., Gotvaldová K., Smolková K., Alán L., Holendová B., Tauber J., Galkin A., Ježek P., Jabůrek M. (2022) Antioxidant role and cardiolipin remodeling by redox-activated mitochondrial Ca²⁺-independent phospholipase A2γ in the brain. Antioxidants, 11, 198. PMID: 35204081 PDF
Ansari F., Yoval-Sánchez B., Niatsetskaya Z., Sosunov S., Stepanova A., Garcia C., Owusu-Ansah E., Ten V., Wittig I., Galkin A. (2021) Quantification of NADH:ubiquinone oxidoreductase (complex I) content in biological samples J.Biol.Chem. 297, 101204. PMID: 34543622 PDF
Sosunov SA, Niatsetskaya ZV, Stepanova AA, Galkin AS, Juliano CE, Ratner VI, Ten VS. (2021) Developmental window of vulnerability to white matter injury driven by sublethal intermittent hypoxemia. Pediatr Res. doi: 10.1038/s41390-021-01555-x. PMID: 33947998 PDF
Jabůrek M, Průchová P, Holendová B, Galkin A, Ježek P. Antioxidant Synergy of Mitochondrial Phospholipase PNPLA8/iPLA2γ with Fatty Acid-Conducting SLC25 Gene Family Transporters. (2021) Antioxidants 10(5). doi: 10.3390/antiox10050678. PMID: 33926059 PDF
Galkin A. (2020) Water route for proton pumping in mitochondrial complex I. Nat.Stuct.Mol.Biol. PMID: 32826994 PDF
Stojakovic A, Trushin S, Sheu A, Khalili L, Chang SY, Li X, Christensen T, Salisbury JL, Geroux RE, Gateno B, Flannery PJ, Dehankar M, Funk CC, Wilkins J, Stepanova A, O’Hagan T, Galkin A, Nesbitt J, Zhu X, Tripathi U, Macura S, Tchkonia T, Pirtskhalava T, Kirkland JL, Kudgus RA, Schoon RA, Reid JM, Yamazaki Y, Kanekiyo T, Zhang S, Nemutlu E, Dzeja P, Jaspersen A, Kwon YIC, Lee MK, Trushina E,. Partial inhibition of mitochondrial complex I ameliorates Alzheimer’s disease pathology and cognition in APP/PS1 female mice. Communications Biology (2021), 4, 61. PMID: 33420340 PDF
Niatsetskaya Z, Sosunov S, Stepanova A, Goldman J, Galkin A, Neginskaya M, Pavlov E, Ten V. (2020) Cyclophilin D-dependent oligodendrocyte mitochondrial ion leak contributes to neonatal white matter injury. J.Clin.Invest. PMID: 32925170 DOI: 10.1172/JCI133082 PDF
Schlegel A, Muller X, Mueller M, Stepanova A, Kron P, de Rougemont O, Muiesan P, Clavien PA, Galkin A, Meierhofer D, Dutkowski P. (2020) Hypothermic oxygenated perfusion protects from mitochondrial injury before liver transplantation. EBioMedicine PMID: 32979838 PDF
Stepanova A, Galkin A. (2020) Measurement of mitochondrial H2O2 production under varying O2 tensions. Methods Cell.Biol. 155, 273-293. PMID: 32183962 PDF
Galkin A (2019) Brain Ischemia/Reperfusion Injury and Mitochondrial Complex I Damage. Biochemistry (Moscow), 84, 1411-1423. PMID: 31760927 PDF
Ten V and Galkin A. (2019) Mechanism of mitochondrial complex I damage in brain ischemia/reperfusion injury. A hypothesis. Mol Cell Neurosci. 100, 103408. PMID: 31494262 PDF
Jain IH, Zazzeron L, Goldberger O, Marutani E, Wojtkiewicz GR, Ast T, Wang H, Schleifer G, Stepanova A, Brepoels K, Schoonjans L, Carmeliet P, Galkin A, Ichinose F, Zapol WM, Mootha VK. (2019) Leigh Syndrome Mouse Model Can Be Rescued by Interventions that Normalize Brain Hyperoxia, but Not HIF Activation. Cell Metab. PMID: 31402314 PDF
Nihori M, Eccles CA, Kurdyukov S, Zemskova M, Varghese MV, Stepanova AA, Galkin A, Rafikov R, Rafikova O. (2019) Rats with Human Mutation of NFU1 Develop Pulmonary Hypertension. Am. J. Respir. Cell. Mol. Biol. PMID: 31461310 PDF
Wojtovich AP, Berry BJ, Galkin A. (2019) Redox Signaling Through Compartmentalization of Reactive Oxygen Species: Implications for Health and Disease. Antioxid Redox Signal. 31, 591-593 PMID: 31084372 PDF
Hayes L, Asressa S, Li Y, Galkin A, Stepanova A, Kawamata H, Manfredi G, Glass J (2019) Distal denervation in the SOD1 knockout mouse correlates with loss of mitochondria at the motor nerve terminal. Experimental Neurology, 318, 251-257, PMID: 31082391 PDF
Stepanova A, Sosunov S, Niatsetskaya Z, Konrad C, Starkov AA, Manfredi G, Wittig I, Ten V, Galkin A. (2019) Redox-dependent loss of flavin by mitochondrial complex I in brain ischemia/reperfusion injury. Antioxid Redox Signal. 31, 608-622. PMID: 31037949 PDF
Stepanova A, Konrad C, Manfredi G, Springett R, Ten V, Galkin A (2019) The dependence of brain mitochondria reactive oxygen species production on oxygen level is linear, except when inhibited by antimycin A. J Neurochem. 148, 731-745, PMID: 30582748 PDF
Kim M, Stepanova A, Niatsetskaya Z, Sosunov S, Arndt S, Murphy MP, Galkin A, Ten V (2018) Attenuation of oxidative damage by targeting mitochondrial complex I in neonatal hypoxic-ischemic brain injury. Free Radic Biol Med. 124, 517-524. PMID: 30037775 PDF
Kahl A, Stepanova A, Konrad C, Anderson C, Manfredi G, Zhou P, Iadecola C, Galkin A. (2018) Critical role of flavin and glutathione in Complex I-mediated bioenergetic failure in brain ischemia/reperfusion injury. Stroke, 49, 223-1231.PMID: 29643256 PDF
Sahni PV, Zhang J, Sosunov S, Galkin A, Niatsetskaya Z, Starkov A, Brookes PS, Ten VS. (2018) Krebs cycle metabolites and preferential succinate oxidation following neonatal hypoxic-ischemic brain injury in mice. Pediatric research, 83, 491-497. PMID: 29211056 PDF
Stepanova A, Konrad C, Guerrero-Castillo S, Manfredi G, Vannucci S, Arnold S, Galkin A. (2018) Deactivation of mitochondrial complex I after hypoxia-ischemia in the immature brain. J.Cerebral Blood Flow and Metabolism. 271678X18770331. PMID:29629602 PDF
Sansone P, Savini C, Kurelac I, Chang Q, Amato L, Strillacci A, Stepanova A, Iommarini L, Mastroleo C, Daly L, Galkin A, Thakur B, Soplop N, Uryu K, Hoshino A, Norton L, Bonafe M, Cricca M, Gasparre G, Lyden D, Bromberg J. (2017) Packaging and transfer of mtDNA via exosomes regulate escape from dormancy in hormonal therapy resistant breast cancer Proc Natl Acad Sci U S A, 114:E9066-E9075. PMID: 29073103 PDF
Stepanova, A., Kahl, A., Konrad, C., Ten, V., Starkov, A, Galkin, A. (2017) Reverse electron transfer results in a loss of flavin from mitochondrial complex I. Potential mechanism for brain ischemia/reperfusion injury, J.Cerebral Blood Flow and Metabolism. 271678 X 17730242. PMID:28914132 PDF
Lin H, Magrane J, Rattelle A, Stepanova A, Galkin A, Clark E, Dong Y, M. Halawani S, Lynch D. (2017) Early cerebellar deficits in mitochondrial biogenesis and respiratory chain complexes in the KIKO mouse model of Friedreich ataxia. Disease Models & Mechanisms, 10:1343-1352. PMID:29125827 PDF
Valsecchi F, Konrad C, D’Aurelio M, Ramos-Espiritu LS, Stepanova A, Burstein SR, Galkin A, Magranè J, Starkov A, Buck J, Levin LR, Manfredi G. (2017) Distinct intracellular sAC-cAMP domains regulate ER calcium signaling and OXPHOS function. J. Cell. Sci. 130:3713-3727. PMID:28864766 PDF
Suslova TA, Burmistrova AL, Vavilov MN, Chernova MS, Khromova EB, Belyaeva SV, Zaripova ON, Stashkevich DS, Galkin A, Darke C. (2017) HLA gene and haplotype frequencies in a Nagaybaks population from the Chelyabinsk Region (Russian South Urals). Hum. Immunol. 78:595-601. PMID:28844894 PDF
Kiprowska M.J., Stepanova A., Todaro D.R., Galkin A., Haas A., Wilson S.M., Figueiredo-Pereira M.E.. (2017) Neurotoxic mechanisms by which the USP14 inhibitor IU1 depletes ubiquitinated proteins and Tau in rat cerebral cortical neurons: relevance to Alzheimer’s disease. Biochim Biophys Acta., 1863:1157-1170. PMID:28372990 PDF
Galkin A., Moncada S. (2017) Modulation of the conformational state of mitochondrial complex I as a target for therapeutic intervention. Interface Focus, 7:20160104. PMID:28382200 PDF
Stepanova A, Shurubor Y, Valsecchi F, Manfredi G, Galkin A. (2016) Differential susceptibility of mitochondrial complex II to inhibition by oxaloacetate in brain and heart. Biochim Biophys Acta. 1857:1561-1568. PMID: 27287543 PDF
Dröse, S., Stepanova A., Galkin A. (2016) Ischemic A/D transition of mitochondrial complex I and its role in ROS generation. Biochim. Biophys. Acta. 1857, 946-957, PMID: 26777588 PDF
Hyspler R., Ticha A., Schierbeek H., Galkin A., Zadak Z. (2015) The evaluation and quantitation of dihydrogen metabolism using deuterium isotope in rats. PLoS ONE 10:e0130687. PMID:26103048 PDF
Stepanova A., Valls A., Galkin A. (2015) Effect of monovalent cations on the kinetics of hypoxic conformational change of mitochondrial complex I. Biochim. Biophys. Acta. 1847:1085–1092. PMID:26009015 PDF
Glebova K., Reznik O., Reznik A., Mehta R., Galkin A., Baranova A., Skoblov M. (2014) A potential of siRNA technology in kidney transplantation, BioDrugs 28:345-361 PMID:24573958 PDF
Babot M., Labarbuta P., Birch A., Kew S., Fuszard M., Botting C., Heide H., Wittig I., Galkin A. (2014) ND3, ND1 and 39 kDa subunits are more exposed in the de-active form of bovine mitochondrial complex I. Biochim. Biophys. Acta. 1837:929-939. PMID:24560811 PDF
Babot M., Labarbuta P., Birch A., Galkin A. (2014) Characterisation of the active/deactive transition of mitochondrial complex I. Biochim. Biophys. Acta. 1837:1083-1092. PMID:24569053 PDF
Babot M., Galkin A. (2013) Molecular mechanism and physiological role of active-deactive transition of mitochondrial complex I. Biochem. Soc. Trans. 41:1325-1330. PMID:24059527 PDF
Ciano M., Fuszard M., Heide H., Botting C.H., Galkin A. (2013) Conformation-specific crosslinking of mitochondrial complex I. FEBS Lett. 587:867-872. PMID:23454639 PDF
Gorenkova N., Robinson E., Grieve D., Galkin A. (2013) Conformational change of mitochondrial complex I increases ROS sensitivity during ischaemia. Antioxid Redox Signal. 19, 1459-68. PMID:23419200 PDF
Carey N., Galkin A., Henriksson P., Richards J.G., Sigwart J.D (2013) Variation in oxygen consumption among ‘living fossils’ (Mollusca: Polyplacophora) J Mar Biol Assoc U.K. 93, 197–207. DOI:10.1017/S0025315412000653 PDF
Gostimskaya I., Galkin A. (2010) Preparation of Highly Coupled Rat Heart Mitochondria. Journal of Visual Experiment, DOI: 10.3791/2202I. PMID: 20972393 PDF
Galkin, A., Abramov, A., Frakich, N., Duchen, M., Moncada, S. (2009) Lack of oxygen deactivates mitochondrial complex I: Implications for ischemic injury? J.Biol.Chem., 284: 36055-36061. PMID:19861410 PDF
Drose S., Galkin A., Brandt, U. (2009). Measurement of superoxide formation by mitochondrial complex I of Yarrowia lipolytica. Methods in Enzymology, 456: 475-490. PMID:19348905 PDF
Galkin, A., Meyer, B., Wittig, I., Karas, M., Schägger H., Vinogradov, A., Brandt, U. (2008) Identification of the mitochondrial complex I ND3 subunit as a structural component involved in the active/de-active enzyme transition. J.Biol.Chem., 283: 20907-20913. PMID:18502755 PDF
Galkin, A. and Moncada, S. (2007 )Nitrosation of mitochondrial complex I. J.Biol.Chem., 282: 37448-37453. PMID:17956863 PDF
Galkin, A., Higgs, A., Moncada, S. (2007) Nitric oxide and hypoxia. Essays in Biochemistry, 43: 29-40. PMID:17705791 PDF
Galkin, A., Dröse, S., Brandt, U. (2006) The proton pumping stoichiometry of purified mitochondrial complex I reconstituted into proteoliposomes. Biochim. Biophys. Acta., 1757:1575-1581. PMID:17094937 PDF
Galkin, A., Zwicker, K., Dröse, S., Grgic, L., Kerscher, S. and Brandt, U. (2006) The redox-Bohr group associated with iron-sulfur cluster N2 of complex I. J.Biol.Chem., 281:23013-23017. PMID:16760472 PDF
Dröse, S., Galkin, A., Brandt, U. (2005) Proton pumping by complex I (NADH:ubiquinone oxidoreductase) from Yarrowia lipolytica reconstituted into proteoliposomes. Biochim. Biophys. Acta., 1710:87-95. PMID:16289468 PDF
Galkin, A. and Brandt, U. (2005) Superoxide radical formation by pure complex I (NADH: Ubiquinone oxidoreductase) from Yarrowia lipolytica. J.Biol.Chem., 280:30129-30135. PMID:15985426 PDF
Brandt, U., Abdrakhmanova, A., Zickermann, V., Galkin, A., Dröse, S., Zwicker, K., Kerscher, S. (2005) Structure-function relationship in mitochondrial complex I of the strictly aerobic yeast Yarrowia lipolytica. Biochem.Soc.Trans., 33:840-844. PMID:16042611 PDF
Eschemann A., Galkin, A., Oettmeier, W., Brandt, U., Kerscher, S. (2005) HDQ (1-hydroxy-2-dodecyl-4(1H)-quinolone), a high affinity inhibitor for mitochondrial alternative NADH-dehydrogenase – evidence for a ping-pong mechanism. J.Biol.Chem., 280:3138-3142. PMID:15533932 PDF
Finikova O., Galkin, A., Cordero, M., Hägerhäll, C., Vinogradov, S. (2003) Dendritic porphyrins and tetrabenzoporphyrins: tunable membrane-impermeable fluorescent pH sensors. J.Am.Chem.Soc., 125:4882 – 4893. PMID:12696908 PDF
Galkin, A., Grivennikova, V.G., Vinogradov, A.D. (2001) Stoichiometry of the NADH:Ubiquinone reductase reaction catalyzed by mitochondrial Complex I. Biochemistry (Moscow), 66:435-537. PMID:11403652 PDF
Galkin, A., Grivennikova, V.G., Vinogradov, A.D. (1999) Stoichiometry in NADH-quinone reductase reactions catalyzed by the bovine heart submitochondrial particles. FEBS Lett., 451:157-161. PMID:10371157 PDF